CD28 抗体
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北京 101111
Quick Overview for CD28 抗体 (ABIN2689147)
抗原
See all CD28 抗体适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 Molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells. This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, rhesus and cynomolgus macaques. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes, however, as described in the literature, the frequency of CD28.2-positive lymphocytes is reduced in the non-human primate, with the majority of cells being CD28 negative. Profile of anti-CD28 reactivity on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta) analyzed by flow cytometry.
BD Pharmingen™ Purified Mouse Anti-Human CD28 - Purified - Clone CD28.2 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human CD28 Transfected Cell Line
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亚型
- IgG1 kappa
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (AA 170-220) antibodyCD28 适用: 人, 小鼠 FACS, WB, IP, IHC, ICC 宿主: 兔 Monoclonal BLR200J unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) (AA 19-140) antibodyCD28 适用: 人 IHC, ICC 宿主: 兔 Monoclonal BLR199J unconjugated Recombinant Antibody
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM63 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, ICC, IHC (fro) 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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- CD28
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别名
- CD28
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背景
- Synonyms: CD28 antigen, T44, Tp44, TP44
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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