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2B4 抗体

Name: 2B4 抗体
SKU: ABIN2689113
Availability: OutOfStock

Cited in 13+ publications. This 小鼠单克隆 anti-2B4 antibody (Clone 2B4) specifically detects 2B4 in FACS, IP, IHC (p) 和 IHC (fro). The antibody is reactive with 小鼠 samples.

(13 references)

产品编号 ABIN2689113

发货至: 中国

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Quick Overview for 2B4 抗体 (ABIN2689113)

抗原

See all 2B4 (CD244) 抗体

2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

适用

小鼠

宿主

小鼠

克隆类型

单克隆

标记

This 2B4 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

2B4

Discover our top product CD244 Primary Antibody

产品特性

CD244 is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF). It is expressed on all natural killer (NK) cells, IL-2-activated NK (LAK) cells, committed progenitors of NK cells, and a subset of T lymphocytes which mediate non-MHC-restricted cytoxicity, including dendritic epidermal T cells. The 2B4 antibody reacts with CD244.2, the 2B4 alloantigen which is expressed on C57BL/6 and C58/J mice, but not in most strains tested (A/J, AKR, BALB/c, CBA/J, CBA/N, C3H/He, C57BR, DBA/1, DBA/2, NZB, SJL/J, 129). At least two isoforms of CD244.2 protein, products of alternative splicing of hnRNA, are expressed on IL-2-activated C57BL/6 NK cells. They differ only in their cytoplasmic domains, with 2B4L (150-aa cytoplasmic tail) having inhibitory activity and 2B4S (93-aa tail) being stimulatory. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. 2B4 antibody activates lytic and secretory functions of IL-2-cultured NK cells and CD244.2+ T cells. Two-color analysis of CD244.2 expression on LAK cells. C57BL/6 LAK cells were simultaneously stained with PE-conjugated PK136 (anti-mouse NK-1.1, Cat. No. 557391/553165, both panels) and purified 2B4 (right panel) monoclonal antibodies, followed by FITC-conjugated anti-mouse IgG2b mAb R12-3 (Cat. No. 553395, both panels). Flow cytometry was performed on a BD FACScan™ System .

BD Pharmingen™ Purified Mouse Anti-Mouse CD244.2 - Purified - Clone 2B4 - Isotype Mouse IgG2b, κ - Reactivity Ms - 0.1 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

rIL-2-propagated NK1.1+ cells from C57BL/6 mice

亚型

IgG2b kappa
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 20-221) antibody
CD244 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) antibody
CD244 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM69 unconjugated Recombinant Antibody

anti-Natural Killer Cell Receptor 2B4 (CD244) (N-Term) antibody
CD244 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 151-250) antibody
CD244 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) (pTyr271) antibody
CD244 适用: 人 WB, ELISA, FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 26-212) antibody
CD244 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-224) antibody
CD244 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-224) antibody
CD244 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) antibody
CD244 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Natural Killer Cell Receptor 2B4 (CD244) antibody
CD244 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB8095 unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Rosmaraki, Douagi, Roth, Colucci, Cumano, Di Santo: "Identification of committed NK cell progenitors in adult murine bone marrow." in: European journal of immunology, Vol. 31, Issue 6, pp. 1900-9, (2001) (PubMed).

Kumaresan, Stepp, Bennett, Kumar, Mathew: "Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4." in: Immunogenetics, Vol. 51, Issue 4-5, pp. 306-13, (2000) (PubMed).

Brown, Boles, van der Merwe, Kumar, Mathew, Barclay: "2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48." in: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2083-90, (1999) (PubMed).

Idris, Smith, Mason, Ortaldo, Scalzo, Yokoyama: "The natural killer gene complex genetic locus Chok encodes Ly-49D, a target recognition receptor that activates natural killing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 11, pp. 6330-5, (1999) (PubMed).

Kubota, Katoh, Muguruma, Koyama: "Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen." in: Immunology, Vol. 96, Issue 3, pp. 491-7, (1999) (PubMed).

Schatzle, Sheu, Stepp, Mathew, Bennett, Kumar: "Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3870-5, (1999) (PubMed).

Stepp, Schatzle, Bennett, Kumar, Mathew: "Gene structure of the murine NK cell receptor 2B4: presence of two alternatively spliced isoforms with distinct cytoplasmic domains." in: European journal of immunology, Vol. 29, Issue 8, pp. 2392-9, (1999) (PubMed).

Latchman, McKay, Reiser: "Identification of the 2B4 molecule as a counter-receptor for CD48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5809-12, (1998) (PubMed).

Davis, van der Merwe: "The structure and ligand interactions of CD2: implications for T-cell function." in: Immunology today, Vol. 17, Issue 4, pp. 177-87, (1997) (PubMed).

Schuhmachers, Ariizumi, Mathew, Bennett, Kumar, Takashima: "2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 592-6, (1995) (PubMed).

Schuhmachers, Ariizumi, Mathew, Bennett, Kumar, Takashima: "Activation of murine epidermal gamma delta T cells through surface 2B4." in: European journal of immunology, Vol. 25, Issue 4, pp. 1117-20, (1995) (PubMed).

Mathew, Garni-Wagner, Land, Takashima, Stoneman, Bennett, Kumar: "Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5328-37, (1993) (PubMed).

Sentman, Hackett, Moore, Tutt, Bennett, Kumar: "Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen." in: Hybridoma, Vol. 8, Issue 6, pp. 605-14, (1990) (PubMed).
抗原

2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

别名

CD244.2

背景

Synonyms: 2B4 B6 Alloantigen