2B4 抗体
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北京 101111
Quick Overview for 2B4 抗体 (ABIN2689112)
抗原
See all 2B4 (CD244) 抗体适用
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克隆类型
标记
应用范围
克隆位点
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产品特性
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CD244 (also known as 2B4 Antigen) is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).3,4,5 It is expressed on all natural killer (NK) cells, IL-2-activated NK cells (LAK), and a subset of T lymphocytes which mediate non-MHC-restricted cytotoxicity. The C9.1 antibody reacts with CD244.1, the 2B4 alloantigen which is expressed in most strains tested (A/J, AKR/N, BALB/c, CBA/N, C3H/He, DBA/1, DBA/2, NZB/N, SJL/J, 129/Svj), but not C57BL/6 or C58/J mice. The expression of the CD244.1 alloantigen on B lymphocytes that was originally described, has not been confirmed in later studies. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. Two-color analysis of CD244.1 expression on splenic NK cells. BALB/c splenocytes were stained with either purified rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553986, left panel) or purified mAb C9.1 (right panel), followed by FITC-conjugated anti-rat IgG2b mAb RG7/11.1 (Cat. No. 553900) then PE-conjugated anti-mouse CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse CD244.1 - Purified - Clone C9.1 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse MHC-unrestricted killer hybridoma 7D4
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亚型
- IgG2b kappa
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anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 20-221) antibody
CD244 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) antibodyCD244 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM69 unconjugated Recombinant Antibody
anti-Natural Killer Cell Receptor 2B4 (CD244) (N-Term) antibodyCD244 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 151-250) antibodyCD244 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (pTyr271) antibodyCD244 适用: 人 WB, ELISA, FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 26-212) antibodyCD244 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-224) antibodyCD244 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) (AA 22-224) antibodyCD244 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) antibodyCD244 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Natural Killer Cell Receptor 2B4 (CD244) antibodyCD244 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB8095 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Polymorphism in the 2B4 gene of inbred mouse strains." in: Immunogenetics, Vol. 51, Issue 8-9, pp. 758-61, (2000) (PubMed).
: "Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen." in: Immunology, Vol. 96, Issue 3, pp. 491-7, (1999) (PubMed).
: "2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48." in: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2083-90, (1999) (PubMed).
: "Identification of the 2B4 molecule as a counter-receptor for CD48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5809-12, (1998) (PubMed).
: "A killer cell protective antigen expressed by MHC-unrestricted killer hybridomas." in: Cellular immunology, Vol. 181, Issue 1, pp. 50-8, (1997) (PubMed).
: "The structure and ligand interactions of CD2: implications for T-cell function." in: Immunology today, Vol. 17, Issue 4, pp. 177-87, (1997) (PubMed).
: "Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 10, pp. 5328-37, (1993) (PubMed).
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: "Polymorphism in the 2B4 gene of inbred mouse strains." in: Immunogenetics, Vol. 51, Issue 8-9, pp. 758-61, (2000) (PubMed).
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- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
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别名
- CD244.1
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背景
- Synonyms: 2B4 BALB Alloantigen
抗原
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