IL12RB1 抗体 (Biotin)
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Quick Overview for IL12RB1 抗体 (Biotin) (ABIN2689093)
抗原
See all IL12RB1 抗体适用
宿主
克隆类型
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克隆位点
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产品特性
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The 114 antibody reacts with mouse CD212 (the β1 subunit of IL-12Rβ1), originally termed IL-12Rβ, of the mouse IL-12 receptor complex. The IL-12Rβ1 subunit associates with a β2 subunit to form a heterodimeric IL-12 receptor complex. Each one of the IL-12R subunits exhibits low affinity for IL-12, but in combination, they bind IL-12 with high affinity. The IL-12Rβ1 subunit interacts primarily with IL-12 p40 whereas the IL-12Rβ2 binds both to IL-12 p40 and IL-12 p35. IL-12Rβ1 is required for high affinity binding of IL-12 but IL-12Rβ2 is required for signaling. IL-12Rβ1 has more recently been described to bind IL-23, a heterodimer formed of the p40 subunit from IL-12, and p19. The cytoplasmic regions of the β1 and β2 subunits contain the box1 and box2 motifs found in other cytokine receptors such as gp130, LIFR and G-CSFR. IL-12Rβ1 are primarily expressed by activated T cells and NK cells. Experiments with IL-12Rβ1 deficient mice have shown that IL-12Rβ1 is necessary for mouse T and NK cell responsiveness to IL-12 p75. The 114 antibody was generated by immunizing IL-12Rβ1 deficient mice of (129 x BALB/c)F1 background with mouse Ba/F3 cells that were stably transfected with IL-12Rβ1.
BD Pharmingen™ Biotin Mouse Anti-Mouse CD212 - Biotin - Clone 114 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- IL-12Rbeta1 Transfected Cell Line
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亚型
- IgG2a kappa
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anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 420-470) antibody
IL12RB1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) antibodyIL12RB1 适用: 人, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 1-381) antibodyIL12RB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 37-234) antibodyIL12RB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) antibodyIL12RB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 197-348) antibodyIL12RB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 25-305) antibodyIL12RB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 119-280) antibodyIL12RB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (Internal Region) antibodyVerified IL12RB1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 12 Receptor beta 1 (IL12RB1) (AA 1-381) antibodyIL12RB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "IL-12 receptor beta 2 (IL-12R beta 2)-deficient mice are defective in IL-12-mediated signaling despite the presence of high affinity IL-12 binding sites." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6221-8, (2000) (PubMed).
: "Characterization of mouse interleukin-12 p40 homodimer binding to the interleukin-12 receptor subunits." in: European journal of immunology, Vol. 29, Issue 6, pp. 2007-13, (1999) (PubMed).
: "The interleukin-12/interleukin-12-receptor system: role in normal and pathologic immune responses." in: Annual review of immunology, Vol. 16, pp. 495-521, (1998) (PubMed).
: "Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2174-9, (1998) (PubMed).
: "Characterization of IL-12 receptor beta1 chain (IL-12Rbeta1)-deficient mice: IL-12Rbeta1 is an essential component of the functional mouse IL-12 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 4, pp. 1658-65, (1997) (PubMed).
: "A functional interleukin 12 receptor complex is composed of two beta-type cytokine receptor subunits." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 14002-7, (1997) (PubMed).
: "Cloning and characterization of a mouse IL-12 receptor-beta component." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 9, pp. 4286-94, (1995) (PubMed).
: "The alphas, betas, and kinases of cytokine receptor complexes." in: Cell, Vol. 74, Issue 4, pp. 587-90, (1993) (PubMed).
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: "IL-12 receptor beta 2 (IL-12R beta 2)-deficient mice are defective in IL-12-mediated signaling despite the presence of high affinity IL-12 binding sites." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6221-8, (2000) (PubMed).
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- IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))
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别名
- CD212
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背景
- Synonyms: IL-12 Receptor β1 chain
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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