CXCR4 抗体
(10 references)Quick Overview for CXCR4 抗体 (ABIN2689046)
抗原
See all CXCR4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The 2B11/CXCR4 monoclonal antibody specifically reacts with mouse CD184, which is also known as CXC chemokine receptor, CXCR4. CXCR4 (previously known as Fusin and LESTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12. Mouse CXCR4 shows 91 % homology at amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes. Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry. The mouse CXCR4 gene has been mapped to chromosome 1. Detection of CXCR4 expression on BALB/c thymocytes by purified 2B11/CXCR4. BALB/c thymocytes were stained with 0.5 μg/test of Purified Rat anti-Mouse CD184 using 3-step staining protocol outlined below and APC Rat anti-Mouse CD4 (Cat. No. 553051). The level of nonspecific staining was assessed by using Purified Rat IgG2b, κ Isotype Control(Cat. No. 553986). The quadrant markers for the bivariate dot plots were set based on the isotype control. Flow cytometry was performed on a FACScan™ Flow Cytometer .
BD Pharmingen™ Purified Rat Anti-Mouse CD184 (CXCR4) - Purified - Clone 2B11/CXCR4 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- GST-NCXCR4 fusion protein
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亚型
- IgG2b kappa
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anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 201-294) antibody
CXCR4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 1-46) antibodyCXCR4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 262-352) antibodyCXCR4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (N-Term) antibodyCXCR4 适用: 人, 小鼠, 兔, Pig, 马 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 14-40) antibodyCXCR4 适用: 人, 小鼠 WB, ELISA, IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 1-356) antibodyCXCR4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (C-Term) antibodyCXCR4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 1-46) antibodyCXCR4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) antibody (PE)CXCR4 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 12G5 PE
anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) (AA 2-15), (Extracellular) antibodyCXCR4 适用: 人 WB, IHC, FACS, IF, IC, LCI 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells." in: Blood, Vol. 110, Issue 7, pp. 2276-85, (2007) (PubMed).
: "The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 996-1004, (2000) (PubMed).
: "Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1522-31, (1998) (PubMed).
: "Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5455-60, (1997) (PubMed).
: "The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry." in: Nature, Vol. 382, Issue 6594, pp. 829-33, (1997) (PubMed).
: "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 5, pp. 1925-30, (1997) (PubMed).
: "CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons." in: Current biology : CB, Vol. 7, Issue 2, pp. 112-21, (1997) (PubMed).
: "The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1." in: Nature, Vol. 382, Issue 6594, pp. 833-5, (1997) (PubMed).
: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, (1996) (PubMed).
: "Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes." in: The Journal of biological chemistry, Vol. 269, Issue 1, pp. 232-7, (1994) (PubMed).
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: "Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells." in: Blood, Vol. 110, Issue 7, pp. 2276-85, (2007) (PubMed).
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- CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))
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别名
- CD184 CXCR4
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背景
- Synonyms: CXCR4, C-X-C chemokine receptor type 4, Fusin, LESTR, PB-CKR, Sdf1r
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途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events
抗原
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