(9 references)-
- 抗原 See all IGLL1 抗体
- IGLL1 (Immunoglobulin lambda-Like Polypeptide 1 (IGLL1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IGLL1 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked CD179b (λ5) and CD179a (VpreB) proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The LM34 antibody reacts with λ5 and surrogate light chain, but not VpreB alone, in transfected X63-Ag8.653 cells. It detects surrogate light chain on pro-B and pre-B cell lines, in the presence or absence of IgH, but not on IgM-positive B lymphocytes. It also detects surrogate light chain or λ5 on the surface and in the cytoplasm of pro-B or pre-B cells from the bone marrow of normal or RAG2-deficient mice. It has been noted that the (CD45R/B220) cell-surface expression of surrogate light chain is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37 °C. At the earliest stages of B lymphopoiesis, before IgH is available, the surrogate light chain associates with a complex of glycoproteins, including a nonclassical cadherin, which could be involved in selective adhesion events during B-lymphocyte development. After immunization, cell-surface λ5 is detected on a subset of splenic Ig light chain-positive germinal-center B lymphocytes. Two-color analysis of CD179b (?5) expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgMb mAb AF6-78 (Cat. no. 553519), C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37 °C to enhance surrogate light chain expression. Then the leukocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553928, left panel) or biotinylated mAb LM34 (right panel) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142), followed by Streptavidin-APC (Cat. no. 554067) and FITC-conjugated anti-mouse CD45R/B220 mAb 9 RA3-6B2 (Cat. no. 553087/553088). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Biotin Rat Anti-Mouse CD179b - Biotin - Clone LM34 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Purified soluble complex of recombinant mu IgH, lambda5, and V[preB]
- 克隆位点
- LM34
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product IGLL1 Primary Antibody
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anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (AA 1-213) antibody
IGLL1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (AA 29-201) antibodyIGLL1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (AA 1-213) antibodyIGLL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (C-Term) antibodyIGLL1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) antibodyIGLL1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (N-Term) antibodyIGLL1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (AA 64-113) antibodyIGLL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin lambda-Like Polypeptide 1 (IGLL1) (AA 112-206) antibodyIGLL1 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6286-93, (2002) (PubMed).
: "The identification of a nonclassical cadherin expressed during B cell development and its interaction with surrogate light chain." in: The Journal of biological chemistry, Vol. 275, Issue 40, pp. 31134-44, (2000) (PubMed).
: "Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development." in: Immunology, Vol. 101, Issue 4, pp. 435-41, (2000) (PubMed).
: "Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells." in: Immunological reviews, Vol. 175, pp. 33-46, (2000) (PubMed).
: "Antigen receptor engagement turns off the V(D)J recombination machinery in human tonsil B cells." in: The Journal of experimental medicine, Vol. 188, Issue 4, pp. 765-72, (1998) (PubMed).
: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, (1995) (PubMed).
: "Precursor B cells of mouse bone marrow express two different complexes with the surrogate light chain on the surface." in: European journal of immunology, Vol. 25, Issue 2, pp. 446-50, (1995) (PubMed).
: "The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice." in: Cell, Vol. 77, Issue 1, pp. 133-43, (1994) (PubMed).
: "A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 469-78, (1993) (PubMed).
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: "VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6286-93, (2002) (PubMed).
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- 抗原
- IGLL1 (Immunoglobulin lambda-Like Polypeptide 1 (IGLL1))
- 别名
- CD179b (IGLL1 产品)
- 背景
- Synonyms: λ5
- 途径
- Regulation of Cell Size
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