CD16 抗体 (Biotin)
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北京 101111
Quick Overview for CD16 抗体 (Biotin) (ABIN2689005)
抗原
See all CD16 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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The FcG7 antibody reacts with CD16, the transmembrane α chain of Fcγ Receptor III (FcγRIIIAα). In the pig, FcγRIIIAα is expressed on NK cells, monocytes, granulocytes, and pulmonary alveolar macrophages. The FcG7 antibody induces redirected cytotoxic activity of NK cells and granulocytes against tumor cells. Two-color analysis of CD16 expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 (Cat. no. 559586, both panels) and biotinylated mAb FcG7 (Right panel), followed by Streptavidin- FITC (Cat. No. 554060, both panels). Erythrocytes were lysed (PharmLyse™ Lysing Buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via- Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura .
BD Pharmingen™ Biotin Mouse Anti-Pig CD16 - Biotin - Clone FcG7 - Isotype Mouse IgG1, κ - Reactivity Pig - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Pig peripheral blood leukocytes
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亚型
- IgG1 kappa
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anti-CD16 (CD16) antibody
CD16 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Non-Human Primate FACS, IP 宿主: 小鼠 Monoclonal LNK16 unconjugated
anti-CD16 (CD16) antibody (FITC)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal LNK16 FITC
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB, Func 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-168 unconjugated
anti-CD16 (CD16) antibody (Biotin)CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 Biotin
anti-CD16 (CD16) (AA 17-208) antibodyCD16 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2G10A9 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Non-Human Primate FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, 绵羊, Cow, 马 FACS, Func 宿主: 小鼠 Monoclonal KD1 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Characterization of the cytolytic trigger molecules G7/PNK-E as a molecular complex on the surface of porcine phagocytes." in: Cellular immunology, Vol. 161, Issue 2, pp. 270-8, (1995) (PubMed).
: "Molecular cloning and identification of the porcine cytolytic trigger molecule G7 as a Fc gamma RIII alpha (CD16) homologue." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2631-41, (1994) (PubMed).
: "Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7." in: Cellular immunology, Vol. 158, Issue 2, pp. 400-13, (1994) (PubMed).
: "A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes." in: Cellular immunology, Vol. 140, Issue 2, pp. 468-77, (1992) (PubMed).
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: "Characterization of the cytolytic trigger molecules G7/PNK-E as a molecular complex on the surface of porcine phagocytes." in: Cellular immunology, Vol. 161, Issue 2, pp. 270-8, (1995) (PubMed).
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- CD16
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别名
- CD16
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背景
- Synonyms: Fcγ Receptor IIIAα
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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