CD16 抗体
(4 references)Quick Overview for CD16 抗体 (ABIN2689003)
抗原
See all CD16 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both FcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation. Immunohistochemical staining of human CD16 positive cells. Frozen sections of normal human spleen was stained with Purified Mouse Anti-Human CD16 (Cat. No. 550383) antibody. Cells positive for CD16 can be identified by intense brown labeling of their cell membranes. Amplification 20X.
BD Pharmingen™ Purified Mouse Anti-Human CD16 - Purified - Clone 3G8 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 1 mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human polymorphonuclear leukocytes
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亚型
- IgG1 kappa
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anti-CD16 (CD16) antibody
CD16 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Non-Human Primate FACS, IP 宿主: 小鼠 Monoclonal LNK16 unconjugated
anti-CD16 (CD16) antibody (FITC)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal LNK16 FITC
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB, Func 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-168 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibody (Biotin)CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 Biotin
anti-CD16 (CD16) (AA 17-208) antibodyCD16 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2G10A9 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Non-Human Primate FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, 绵羊, Cow, 马 FACS, Func 宿主: 小鼠 Monoclonal KD1 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 125 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "The anti-Fc gamma RIII mAb 3G8 induces neutrophil activation via a cooperative actin of Fc gamma RIIIb and Fc gamma RIIa." in: The international journal of biochemistry & cell biology, Vol. 29, Issue 3, pp. 465-73, (1997) (PubMed).
: "Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain." in: The Journal of experimental medicine, Vol. 175, Issue 5, pp. 1381-90, (1992) (PubMed).
: "Alloimmune neonatal neutropenia due to an antibody to the neutrophil Fc-gamma receptor III with maternal deficiency of CD16 antigen." in: Blood, Vol. 77, Issue 7, pp. 1572-80, (1991) (PubMed).
: "Human neutrophil Fc gamma receptor distribution and structure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 10, pp. 3275-9, (1982) (PubMed).
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: "The anti-Fc gamma RIII mAb 3G8 induces neutrophil activation via a cooperative actin of Fc gamma RIIIb and Fc gamma RIIa." in: The international journal of biochemistry & cell biology, Vol. 29, Issue 3, pp. 465-73, (1997) (PubMed).
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- CD16
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别名
- CD16
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背景
- Synonyms: FcRIII, Fc-gamma RIII, FCG3, FCGR3, FCGRIII, FcγRIII, IGFR3
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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