Syndecan 1 抗体 (Biotin)
(1 reference)Quick Overview for Syndecan 1 抗体 (Biotin) (ABIN2688968)
抗原
See all Syndecan 1 (SDC1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- BD Pharmingen™
-
产品特性
-
The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. Expression of CD138 on mouse bone- arrow B lymphocytes. C57BL/6 bone- arrow leukocytes were simultaneously stained with biotin-conjugated 281-2 mAb (Right panel) and FITC-conjugated RA3-6B2 mAb (anti- D45R/B220, Cat. no. 553087/553088, both panels), followed by Streptavidin-PE (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™
BD Pharmingen™ Biotin Mouse Anti-CD138 - Biotin - Clone 281-2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- NAMRU mouse mammary gland epithelial cell line NMuMG
-
亚型
- IgG2a kappa
-
-
anti-Syndecan 1 (SDC1) (AA 28-171) antibody
SDC1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A3H4 unconjugated
anti-Syndecan 1 (SDC1) (AA 210-238), (C-Term) antibodySDC1 适用: 人 WB, FACS, IF 宿主: 小鼠 Monoclonal 587CT7-3-6-5 unconjugated
anti-Syndecan 1 (SDC1) (AA 23-253) antibodySDC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 58-313) antibodySDC1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 201-310) antibodySDC1 适用: 人, 大鼠, 小鼠, Cow WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 25-240) antibodySDC1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 23-254) antibodySDC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (C-Term) antibodySDC1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 23-254) antibodySDC1 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM45 unconjugated Recombinant Antibody
anti-Syndecan 1 (SDC1) (AA 25-240) antibodySDC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Flow Cytometry System.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
-
: "Changing responsiveness to chemokines allows medullary plasmablasts to leave lymph nodes." in: European journal of immunology, Vol. 31, Issue 2, pp. 609-16, (2001) (PubMed).
-
: "Changing responsiveness to chemokines allows medullary plasmablasts to leave lymph nodes." in: European journal of immunology, Vol. 31, Issue 2, pp. 609-16, (2001) (PubMed).
-
- Syndecan 1 (SDC1)
-
别名
- CD138
-
背景
- Synonyms: Syndecan-1
-
途径
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism
抗原
-
