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- 抗原 See all IL3RA 抗体
- IL3RA (Interleukin 3 Receptor, alpha (IL3RA))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL3RA antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The 5B11 antibody reacts with mouse CD123, the α chain subunit (IL-3Rα) of the mouse IL-3 receptor. The IL-3Rα chain is a 60-70 kD transmembrane glycoprotein that binds IL-3 with low affinity but cannot transduce signals by itself. The mouse IL-3Rα chain can complex with either of two homologous β chain subunits (βc and βIL-3) to form high-affinity heterodimeric IL-3 receptors. The βc chain is the common β chain subunit that can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors. The βIL-3 subunit can form high affinity IL-3 receptor complexes with only the IL-3Rα subunit. The βIL-3 subunit clone is specific for mouse IL-3 and binds with low affinity. The immunogen used to generate the 5B11 hybridoma was CTLL cells transfected with the mouse IL-3R alpha chain. The 5B11 antibody does not block the binding of IL-3 protein to the high affinity IL-3 receptor heterodimer.
BD Pharmingen™ Biotin Rat Anti-Mouse CD123 - Biotin - Clone 5B11 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse IL-3R transfected CTLL cells
- 克隆位点
- 5B11
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product IL3RA Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene." in: The EMBO journal, Vol. 14, Issue 5, pp. 939-50, (1995) (PubMed).
: "Subset of CD4+ T cell clones expressing IL-3 receptor alpha-chains uses IL-3 as a cofactor in autocrine growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 7, pp. 3014-27, (1994) (PubMed).
: "Two distinct functional high affinity receptors for mouse interleukin-3 (IL-3)." in: The EMBO journal, Vol. 11, Issue 5, pp. 1875-84, (1992) (PubMed).
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Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene." in: The EMBO journal, Vol. 14, Issue 5, pp. 939-50, (1995) (PubMed).
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- 抗原
- IL3RA (Interleukin 3 Receptor, alpha (IL3RA))
- 别名
- CD123 (IL3RA 产品)
- 别名
- CD123 antibody, IL3R antibody, IL3RAY antibody, IL3RX antibody, IL3RY antibody, hIL-3Ra antibody, CDw123 antibody, SUT-1 antibody, Cyrl antibody, interleukin 3 receptor subunit alpha antibody, interleukin 3 receptor, alpha chain antibody, IL3RA antibody, Il3ra antibody
- 背景
- Synonyms: IL-3 Receptor α chain
- 途径
- JAK/STAT Signaling
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