IFNGR1 抗体 (Biotin)
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Quick Overview for IFNGR1 抗体 (Biotin) (ABIN2688897)
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See all IFNGR1 抗体适用
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产品特性
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The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon-γ receptor (IFN-γRα, aka, CD119). The functionally active-form of the mouse IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γRα and IFN-γRβ chains required for the transduction of biologic responses. IFN-γRα is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody, it does not block the binding of IFN-γ to its receptor. The immunogen used to generate this hybridoma was a purified preparation of soluble recombinant mouse IFN-γRα chain protein. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of cell surface IFN-γRα by BALB/c splenic lymphocytes. RBC-lysed BALB/c spleen cells were preincubated (~15 minutes, 4 °C) with purified 2.4G2 antibody [rat anti-mouse CD16 (FcγIII)/CD32 (FcγII), Cat. No. 553142, 1 μg antibody/10e6 cells]. The cells were stained (30 minutes, 4 °C) with biotinylated 2E2 antibody (1 μg mAb/10e6 cells, Cat. No. 550482) followed by R-PE-conjugated streptavidin (Cat. No. 554061, 0.015 μg PE-SA/10e6 cells). After washing, the cells were analyzed with a FACScan™ Flow Cytometer. The immunofluorescent staining patterns for cells stained with either biotinylated 2E2 antibody (filled histogram) or Streptavidin-PE (background staining, empty histogram) are shown. The histograms were generated from reanalyzed flow cytometric data files that were gated for events with the light-scattering characteristics of lymphocytes.
BD Pharmingen™ Biotin Hamster Anti-Mouse CD119 - Biotin - Clone 2E2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein
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亚型
- IgG1 kappa
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anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibody
IFNGR1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3E1A9 unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 270-489) antibodyIFNGR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibodyIFNGR1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5H1C9 unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 1-489) antibodyIFNGR1 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) antibodyKO Validated IFNGR1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (C-Term) antibodyIFNGR1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 431-480) antibodyIFNGR1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibodyIFNGR1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) antibodyIFNGR1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 181-193) antibodyVerified IFNGR1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).
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: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).
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- IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))
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别名
- CD119
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背景
- Synonyms: IFN-γ Receptor α chain
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途径
- Interferon-gamma Pathway
抗原
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