IFNGR1 抗体
(3 references)Quick Overview for IFNGR1 抗体 (ABIN2688896)
抗原
See all IFNGR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- BD Pharmingen™
-
产品特性
-
The GIR-94 antibody recognizes the extracellular region of the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα, aka, CD119). The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γR α and β chains required for the transduction of biologic responses. The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα purified from human placenta. The GIR-94 is a non-neutralizing antibody. Human PBMC (Left panel) and red blood cells (Right panel) were isolated by Lymphoprep (Nycomed) density centrifugation. The cells were stained with GIR-94 (1 μg, Cat. No. 558935) followed by biotinylated goat anti- mouse IgG (0.25 μg, Cat. No. 553999) and streptavidin -PE (0.015 μg, Cat No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the GIR-94 antibody (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in left panel are gated on the lymphocyte population defined by its light scattering characteristics.
BD Pharmingen™ Purified Mouse Anti-Human CD119 - Purified - Clone GIR-94 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Human IFN-gammaRalpha
-
亚型
- IgG2b kappa
-
-
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibody
IFNGR1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3E1A9 unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 270-489) antibodyIFNGR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibodyIFNGR1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5H1C9 unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 1-489) antibodyIFNGR1 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) antibodyKO Validated IFNGR1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (C-Term) antibodyIFNGR1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 431-480) antibodyIFNGR1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibodyIFNGR1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 181-193) antibodyVerified IFNGR1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 1-489) antibodyIFNGR1 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "The IFN gamma receptor: a paradigm for cytokine receptor signaling." in: Annual review of immunology, Vol. 15, pp. 563-91, (1997) (PubMed).
: "Interferon-gamma induces receptor dimerization in solution and on cells." in: The Journal of biological chemistry, Vol. 268, Issue 24, pp. 18103-10, (1993) (PubMed).
: "Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4231-7, (1988) (PubMed).
-
: "The IFN gamma receptor: a paradigm for cytokine receptor signaling." in: Annual review of immunology, Vol. 15, pp. 563-91, (1997) (PubMed).
-
- IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))
-
别名
- CD119
-
背景
- Synonyms: IFN-γ Receptor α chain
-
途径
- Interferon-gamma Pathway
抗原
-
