Cardiac Troponin T2 抗体
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Quick Overview for Cardiac Troponin T2 抗体 (ABIN2688865)
抗原
See all Cardiac Troponin T2 (cTnT) 抗体适用
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The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels. Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells. LEFT PANEL: Western blot analysis of Cardiac Troponin T expression in human and mouse tissues. Human heart lysate was obtained from Abcam (Cat .No. ab2943, lane 1). C2C12 mouse myoblasts (ATCC CRL-1772) were cultured in DMEM containing 10 % FBS (lane 2) or 0.5 % FBS for 14 days for induction of cell differentiation (lane 3). Lysates were prepared, electrophoresed (SDS-PAGE) and transferred to PVDF membranes. The membranes were labeled with 1 μg/mL of Purified Mouse Anti-Cardiac Troponin T. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT PANEL: Immunohistochemical staining of Cardiac Troponin T in human heart. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-Cardiac Troponin T (right). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. Flow cytometric analysis of Cardiac Troponin T in human (left panel) and mouse (right panel) cardiomyocytes differentiated in vitro. Human embryonic stem cell-derived cardiomyocytes (Evans lab, UCSD) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885). The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer, and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-Cardiac Troponin T (solid line). The second-step reagents were FITC Goat Anti-Mouse Ig (Cat. No. 554001, left) and PE Goat Anti-Mouse Ig (Cat. No. 550589, right). Events with the forward and side light-scatter characteristics of intact cells were gated. Flow cytometry was performed on a BD LSR Fortessa™ (left) or a BD FACSCanto™ II (right) flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Cardiac Troponin T - Purified - Clone 13-11 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu, Chick, Dog, G Pig, Pig, Rab, Rat - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Rabbit cardiac troponin T Protein
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亚型
- IgG1 kappa
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anti-Cardiac Troponin T (cTnT) (AA 56-271) antibody
cTnT 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin T (cTnT) (AA 1-120) antibodycTnT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin T (cTnT) (AA 1-295) antibodycTnT 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Cardiac Troponin T (cTnT) antibodycTnT 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Cardiac Troponin T (cTnT) antibodycTnT 适用: 小鼠, 大鼠 IHC, IF, IEM 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin T (cTnT) antibodycTnT 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1G1 unconjugated
anti-Cardiac Troponin T (cTnT) (AA 2-100) antibodycTnT 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated
anti-Cardiac Troponin T (cTnT) (AA 138-298) antibodycTnT 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin T (cTnT) (AA 1-289) antibodycTnT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin T (cTnT) (C-Term) antibodycTnT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 61, Issue 2, pp. 169-75, (2012) (PubMed).
: "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. E1848-57, (2012) (PubMed).
: "Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression." in: PLoS ONE, Vol. 6, Issue 8, pp. e23657, (2011) (PubMed).
: "Functional cardiomyocytes derived from human induced pluripotent stem cells." in: Circulation research, Vol. 104, Issue 4, pp. e30-41, (2009) (PubMed).
: "Generation of functional murine cardiac myocytes from induced pluripotent stem cells." in: Circulation, Vol. 118, Issue 5, pp. 507-17, (2008) (PubMed).
: "Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere." in: Cell, Vol. 77, Issue 5, pp. 701-12, (1994) (PubMed).
: "A cardiac troponin T epitope conserved across phyla." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9269-74, (1992) (PubMed).
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: "Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 61, Issue 2, pp. 169-75, (2012) (PubMed).
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- Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))
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别名
- Cardiac Troponin T
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背景
- Synonyms: cTnT, Cardiac Muscle Troponin T, Troponin T type 2, TNNT2
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分子量
- 35-40 kDa
抗原
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