Alkaline Phosphatase 抗体
(5 references)-
- 抗原 See all Alkaline Phosphatase (ALP) 抗体
- Alkaline Phosphatase (ALP)
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Alkaline Phosphatase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), BioImaging (BI)
- 品牌
- BD Pharmingen™
- 产品特性
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The B4-78 monoclonal antibody reacts with the tissue-nonspecific isozyme of alkaline phosphatase. Alkaline phosphatases are membrane-bound glycoproteins. Four isozymes of alkaline phosphatase exist in humans: placental, placental-like, intestinal, and liver/bone/kidney. Liver/bone/kidney alkaline phosphatase is also known as tissue-nonspecific alkaline phosphatase (TNAP). Human embryonic stem cells and embryonic carcinoma cells express high levels of tissue-nonspecific alkaline phosphatase that decrease upon differentiation. Genetic and biochemical studies suggest that TNAP plays a role in skeletal mineralization. Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) grown in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody (solid line) or a Purified mouse IgG1, κ isotype control (
BD Pharmingen™ Purified Mouse anti-Human Alkaline Phosphatase - Purified - Clone B4-78 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Bone Alkaline Phosphatase
- 克隆位点
- B4
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product ALP Primary Antibody
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anti-Alkaline Phosphatase (ALP) (AA 56-83) antibody
ALP 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人, Cow IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 282-309) antibodyALP 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 21-35) antibodyALP 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).
: "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. ..." in: Nature biotechnology, Vol. 25, Issue 7, pp. 803-16, (2007) (PubMed).
: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, (2006) (PubMed).
: "Circulating osteoblast-lineage cells in humans." in: The New England journal of medicine, Vol. 352, Issue 19, pp. 1959-66, (2005) (PubMed).
: "Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells." in: Journal of cellular physiology, Vol. 154, Issue 2, pp. 317-28, (1993) (PubMed).
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: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).
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- 抗原
- Alkaline Phosphatase (ALP)
- 别名
- Alkaline Phosphatase (ALP 产品)
- 别名
- ALP antibody, ALP1 antibody, CRASH antibody, ECK0378 antibody, JW0374 antibody, psiA antibody, 2410004D18Rik antibody, AU040643 antibody, AW060375 antibody, ILC antibody, CTACK antibody, ESkine antibody, Akp2 antibody, alp antibody, zgc:56672 antibody, DDBDRAFT_0205491 antibody, DDBDRAFT_0231570 antibody, DDB_0205491 antibody, DDB_0231570 antibody, Actn2lp antibody, Alp antibody, asparaginase like 1 antibody, bacterial alkaline phosphatase antibody, C-C motif chemokine ligand 27 antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase antibody, PDZ and LIM domain 3 antibody, ASRGL1 antibody, phoA antibody, Asrgl1 antibody, CCL27 antibody, alpl antibody, alp antibody, Pdlim3 antibody
- 背景
- Synonyms: Bone/Kidney/Liver Alkaline Phosphatase, TNAP, TNSALP, AP-TNAP, ALPL Entrez Gene ID: 249
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