Alkaline Phosphatase 抗体
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北京 101111
Quick Overview for Alkaline Phosphatase 抗体 (ABIN2688842)
抗原
See all Alkaline Phosphatase (ALP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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The B4-78 monoclonal antibody reacts with the tissue-nonspecific isozyme of alkaline phosphatase. Alkaline phosphatases are membrane-bound glycoproteins. Four isozymes of alkaline phosphatase exist in humans: placental, placental-like, intestinal, and liver/bone/kidney. Liver/bone/kidney alkaline phosphatase is also known as tissue-nonspecific alkaline phosphatase (TNAP). Human embryonic stem cells and embryonic carcinoma cells express high levels of tissue-nonspecific alkaline phosphatase that decrease upon differentiation. Genetic and biochemical studies suggest that TNAP plays a role in skeletal mineralization. Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) grown in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody (solid line) or a Purified mouse IgG1, κ isotype control (
BD Pharmingen™ Purified Mouse anti-Human Alkaline Phosphatase - Purified - Clone B4-78 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Bone Alkaline Phosphatase
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亚型
- IgG1 kappa
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anti-Alkaline Phosphatase (ALP) (AA 56-83) antibody
ALP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 23GB1765 unconjugated Recombinant Antibody
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 282-309) antibodyALP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人, Cow IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase (ALP) (AA 21-35) antibodyALP 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).
: "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. ..." in: Nature biotechnology, Vol. 25, Issue 7, pp. 803-16, (2007) (PubMed).
: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, (2006) (PubMed).
: "Circulating osteoblast-lineage cells in humans." in: The New England journal of medicine, Vol. 352, Issue 19, pp. 1959-66, (2005) (PubMed).
: "Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells." in: Journal of cellular physiology, Vol. 154, Issue 2, pp. 317-28, (1993) (PubMed).
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: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).
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- Alkaline Phosphatase (ALP)
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别名
- Alkaline Phosphatase
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背景
- Synonyms: Bone/Kidney/Liver Alkaline Phosphatase, TNAP, TNSALP, AP-TNAP, ALPL Entrez Gene ID: 249
抗原
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