电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Alkaline Phosphatase 抗体

This anti-Alkaline Phosphatase antibody is a 小鼠 单克隆 antibody detecting Alkaline Phosphatase in WB, IF, FACS, IP 和 BI. Suitable for 人 和 小鼠. This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN2688842
发货至: 中国

Quick Overview for Alkaline Phosphatase 抗体 (ABIN2688842)

抗原

See all Alkaline Phosphatase (ALP) 抗体
Alkaline Phosphatase (ALP)

适用

  • 75
  • 19
  • 13
  • 12
  • 10
  • 1
  • 1
人, 小鼠

宿主

  • 65
  • 29
  • 2
  • 1
  • 1
小鼠

克隆类型

  • 49
  • 49
单克隆

标记

  • 48
  • 13
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This Alkaline Phosphatase antibody is un-conjugated

应用范围

  • 57
  • 36
  • 21
  • 16
  • 12
  • 11
  • 10
  • 9
  • 7
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), BioImaging (BI)

克隆位点

B4
  • 品牌

    BD Pharmingen™

    产品特性

    The B4-78 monoclonal antibody reacts with the tissue-nonspecific isozyme of alkaline phosphatase. Alkaline phosphatases are membrane-bound glycoproteins. Four isozymes of alkaline phosphatase exist in humans: placental, placental-like, intestinal, and liver/bone/kidney. Liver/bone/kidney alkaline phosphatase is also known as tissue-nonspecific alkaline phosphatase (TNAP). Human embryonic stem cells and embryonic carcinoma cells express high levels of tissue-nonspecific alkaline phosphatase that decrease upon differentiation. Genetic and biochemical studies suggest that TNAP plays a role in skeletal mineralization. Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) grown in mTeSR™1 medium (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody (solid line) or a Purified mouse IgG1, κ isotype control (

    BD Pharmingen™ Purified Mouse anti-Human Alkaline Phosphatase - Purified - Clone B4-78 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg

    纯化方法

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    免疫原

    Human Bone Alkaline Phosphatase

    亚型

    IgG1 kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Aqueous buffered solution containing ≤0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store undiluted at 4°C.
  • OConnor, Kardel, Iosfina, Youssef, Lu, Li, Vercauteren, Nagy, Eaves: "Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 5, pp. 1109-16, (2008) (PubMed).

    Adewumi, Aflatoonian, Ahrlund-Richter, Amit, Andrews, Beighton, Bello, Benvenisty, Berry, Bevan, Blum, Brooking, Chen, Choo, Churchill, Corbel, Damjanov, Draper, Dvorak, Emanuelsson, Fleck, Ford et al.: "Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. ..." in: Nature biotechnology, Vol. 25, Issue 7, pp. 803-16, (2007) (PubMed).

    Karp, Ferreira, Khademhosseini, Kwon, Yeh, Langer: "Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 4, pp. 835-43, (2006) (PubMed).

    Eghbali-Fatourechi, Lamsam, Fraser, Nagel, Riggs, Khosla: "Circulating osteoblast-lineage cells in humans." in: The New England journal of medicine, Vol. 352, Issue 19, pp. 1959-66, (2005) (PubMed).

    Dorheim, Sullivan, Dandapani, Wu, Hudson, Segarini, Rosen, Aulthouse, Gimble: "Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells." in: Journal of cellular physiology, Vol. 154, Issue 2, pp. 317-28, (1993) (PubMed).

  • 抗原

    Alkaline Phosphatase (ALP)

    别名

    Alkaline Phosphatase

    背景

    Synonyms: Bone/Kidney/Liver Alkaline Phosphatase, TNAP, TNSALP, AP-TNAP, ALPL Entrez Gene ID: 249
You are here:
Chat with us!