A2B5 抗体
适用: 小鸡, 人, 小鼠, 大鼠
FACS, IF, BI, CyTox
宿主: 小鼠
Monoclonal
105-A2B5
unconjugated
(2 references)-
- 抗原
- A2B5
- 适用
- 小鸡, 人, 小鼠, 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI), Cytotoxicity Test (CyTox)
- 品牌
- BD Pharmingen™
- 产品特性
-
The 105/A2B5 monoclonal antibody binds to gangliosides in neural tissue in the retina, brain, spinal cord, and dorsal root ganglia. The antigenic gangliosides are found on the plasma membrane of neural cell bodies, but not axons or dendrites. Expression levels of the gangliosides that mAb 105/A2B5 binds to, including GT3 and its O-acetylated derivative, decrease during embryonic development of the brain. Thus, mAb 105/A2B5 may be used to monitor the maturation of brain tissue. A2B5 antigens have also been associated with numerous tumors, including glioblastoma multiforme tumor cells, pancreatic islet tumor cells, and rat insulinoma cells, and are thought to contribute to chemoresistance and increased tumor proliferation and recurrence. Flow cytometric analysis of A2B5 expression on mouse pancreatic tumor (insulinoma) cells and Human embryonic stem cell (hESC)-derived neurons. Mouse Beta-TC-6 cells (ATCC, CRL-11506, left panel) or human H9 (WiCell, Madison, WI) hESC-derived neurons (right panel) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgM, κ Isotype Control (Cat. No. 555581, dashed line histograms) or Purified Mouse Anti- A2B5 monoclonal antibody (solid line histograms) at matched concentrations. Dot plots were derived from gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-A2B5 - Purified - Clone 105/A2B5 (also known as antibody A2B5, F12A2B5, 105) - Isotype Mouse IgM - Reactivity Ms, Hu, Chick, Rat - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Chicken embryo retina Cells
- 克隆位点
- 105-A2B5
- 亚型
- IgM
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
-
-
: "Flow cytometry analysis of neural differentiation markers expression in human glioblastomas may predict their response to chemotherapy." in: Cellular and molecular neurobiology, Vol. 29, Issue 6-7, pp. 845-58, (2009) (PubMed).
: "Monoclonal antibody to a plasma membrane antigen of neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, Issue 10, pp. 4913-7, (1980) (PubMed).
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: "Flow cytometry analysis of neural differentiation markers expression in human glioblastomas may predict their response to chemotherapy." in: Cellular and molecular neurobiology, Vol. 29, Issue 6-7, pp. 845-58, (2009) (PubMed).
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- 抗原
- A2B5
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