(2 references)-
- 抗原
- O-GlcNAc
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适用
- 化学剂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 纯化方法
- IgM purification
- 免疫原
- This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and threonine.
- 克隆位点
- CTD110-6
- 亚型
- IgM
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anti-O-GlcNAc antibody (HRP)
WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 HRP
anti-O-GlcNAc antibodyWB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 unconjugated
anti-O-GlcNAc antibody (FITC)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 FITC
anti-O-GlcNAc antibody (Alkaline Phosphatase (AP))WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 Alkaline Phosphatase (AP)
anti-O-GlcNAc antibody (Biotin)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 Biotin
anti-O-GlcNAc antibody (PE)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 PE
anti-O-GlcNAc antibody (PerCP)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 PerCP
anti-O-GlcNAc antibody (APC)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 APC
anti-O-GlcNAc antibody (Atto 488)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 Atto 488
anti-O-GlcNAc antibody (Atto 594)WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 9H6 Atto 594
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 1 μg/μL
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -20 °C
- 储存方法
- Antibodies in solution can be stored at -20 °C for 2 years.
- 有效期
- 6 months
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: "Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." in: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).
: "Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine." in: Analytical biochemistry, Vol. 293, Issue 2, pp. 169-77, (2001) (PubMed).
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: "Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc." in: Nature chemical biology, Vol. 6, Issue 5, pp. 338-43, (2010) (PubMed).
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- 抗原
- O-GlcNAc
- 物质类
- Viral Protein
- 背景
- O-GlcNAc (b-O-linked N-acetylglucosamine) is a ubiquitous and dynamic post-translational modification present on numerous cytoplasmic and nuclear proteins and is thought to play a regulatory role in numerous cellular processes including homeostatic mechanisms and gene expression. OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residues whereas OGA / O-GlcNAcase (O- linked N-acetylglucosamine (GlcNAc)-ase) is a glycosidase that removes O-GlcNAc. O-GlcNAc modifications have been described to directly regulate the activities of a variety of transcription factors including Sp1, estrogen receptors, STAT5, NF-κB, p53, YY1, Elf-1, c-Myc, Rb, PDX-1, CREB, fork-head, and others. In addition, RNA polymerase II and the basal transcription factors are also extensively modified by O-GlcNAc. O-GlcNAc modification has been reported to suppress or enhance transcription, depending on the promoter and other associated proteins.
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