MBD3 抗体
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北京 101111
Quick Overview for MBD3 抗体 (ABIN2668686)
抗原
See all MBD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MBD3 antibody (mAb) (Clone 106B691)
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纯化方法
- Affinity Purified
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免疫原
- This MBD3 antibody was raised against a synthetic peptide corresponding to human MBD3.
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亚型
- IgG
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anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 79-209) antibody
MBD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB695 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB10 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) antibodyMBD3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 70-179) antibodyMBD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-291) antibodyMBD3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibodyMBD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 265-291) antibodyMBD3 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 264-291), (C-Term) antibodyMBD3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02375 unconjugated
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-255) antibodyMBD3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1 - 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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浓度
- 0.5 μg/μL
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缓冲液
- PBS containing 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- 4 °C,-20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
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别名
- MBD3
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背景
- MBD3 (methyl-CpG binding domain protein 3) is a subunit of the NuRD, a multi-subunit complex containing nucleosome remodeling and histone deacetylase activities. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD3 regulates transcription by forming a Mi-2/NuRD complex with nucleosome remodeling and histone deacetylase (HDAC) activities in mammalian cells. MBD3 has been shown to interact not with 5-methylcytidine methylated DNA but with DNA containing 5-hydroxymethylcytosine (5-hmC) methylation. MDB3 co-localizes with TET1 and is required for normal levels of 5-hmC in embryonic stem cells.
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分子量
- 50 kDa
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基因ID
- 53615
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NCBI登录号
- NP_001268382
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途径
- Chromatin Binding
抗原
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