CHD5 抗体 (C-Term)
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Quick Overview for CHD5 抗体 (C-Term) (ABIN2668553)
抗原
See all CHD5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Chd5 antibody (mAb) (Clone 5A10)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a peptide within the C-terminal region of mouse Chd5.
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亚型
- IgG2a
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应用备注
- ICC/IF: 2 - 5 μg/mL dilution WB*: 1 - 2 μg/mL dilution The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- CHD5 (Chromodomain Helicase DNA Binding Protein 5 (CHD5))
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别名
- Chd5
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背景
- Chd5 (Chromodomain Helicase DNA Binding Protein 5) is member of the CHD subfamily of chromatin remodeling SWI/SNF proteins, containing chromodomains and a SNF2-like ATPase/helicase domain, that play a role in chromatin remodeling and gene transcription. Chd5 is also a potential tumor suppressor that may play a role in neuronal development.
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分子量
- 260 kDa
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基因ID
- 269610
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NCBI登录号
- NP_001074845
抗原
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