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Rpb1 CTD 抗体 (pSer5, Ser5)

Cited in 5+ publications. This 大鼠 单克隆 anti-Rpb1 CTD antibody (Clone 3E8) specifically detects Rpb1 CTD in WB, IF, ICC, ChIP, ChIP-seq 和 IP. The antibody is reactive with 人 samples.
产品编号 ABIN2668541
发货至: 中国
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Quick Overview for Rpb1 CTD 抗体 (pSer5, Ser5) (ABIN2668541)

抗原

Rpb1 CTD (RNA Polymerase II CTD)

适用

  • 23
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宿主

  • 10
  • 9
  • 4
大鼠

克隆类型

  • 14
  • 9
单克隆

标记

  • 23
This Rpb1 CTD antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

克隆位点

3E8
  • 抗原表位

    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
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    • 1
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    pSer5, Ser5

    纯化方法

    None

    免疫原

    This RNA pol II CTD pSer5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

    亚型

    IgG2a
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    1 μg/μL

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    储存条件

    -80 °C

    储存方法

    Antibodies in solution can be stored at -80 °C for 2 years.

    有效期

    6 months
  • Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

    Ramos, Wijetunga, McLellan, Suzuki, Greally: "DNA demethylation by 5-aza-2'-deoxycytidine is imprinted, targeted to euchromatin, and has limited transcriptional consequences." in: Epigenetics & chromatin, Vol. 8, pp. 11, (2015) (PubMed).

    Eifler, Shao, Bartholomeeusen, Fujinaga, Jäger, Johnson, Luo, Krogan, Peterlin: "Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 468-78, (2014) (PubMed).

    Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

    Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

  • 抗原

    Rpb1 CTD (RNA Polymerase II CTD)

    别名

    RNA Pol II CTD

    分子量

    240 kDa

    基因ID

    5430
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