Histone H3.1 抗体 (H3.1S28ph)
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北京 101111
Quick Overview for Histone H3.1 抗体 (H3.1S28ph) (ABIN2667909)
抗原
See all Histone H3.1 (HIST1H3B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- H3.1S28ph
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原理
- Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a peptide corresponding to phospho-serine 28 of human Histone H3.1. This antibody does not react with Histone H3.3 phosphorylated on serine 28.
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亚型
- IgG2b
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anti-Histone H3.1 (HIST1H3B) (pSer10) antibody
HIST1H3B 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, IHC, FACS 宿主: 兔 Monoclonal 27F2 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3S10ph) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (H3K4me2) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC, FACS 宿主: 兔 Monoclonal 6F6 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (meArg17) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 3E10 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3S10ph) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 2A5 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3R2me1) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 4G5 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (meLys36) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 1F4 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3K14ac) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 3H3 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3K18me1) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 2B5 unconjugated Recombinant Antibody
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应用备注
- ICC/IF: 2 - 10 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- Histone H3.1 (HIST1H3B)
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别名
- Histone H3.1
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背景
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.3 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.3 has a serine. Phosphorylation of serine 28 occurs in early mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells.
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分子量
- 17 kDa
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基因ID
- 8352
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NCBI登录号
- NP_003522
抗原
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