HDAC4 抗体 (AA 194-209)
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北京 101111
Quick Overview for HDAC4 抗体 (AA 194-209) (ABIN2668313)
抗原
See all HDAC4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 194-209
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原理
- HDAC4 antibody (pAb)
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纯化方法
- Affinity Purified
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免疫原
- This HDAC4 antibody was raised against a synthetic peptide corresponding to amino acid residues 194-209 of human HDAC4.
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亚型
- IgG
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anti-Histone Deacetylase 4 (HDAC4) (AA 551-648) antibody
HDAC4 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 456-592) antibodyHDAC4 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E4A5 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 456-592) antibodyHDAC4 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E2E6 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 904-1076) antibodyHDAC4 适用: 小鼠 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 4-213) antibodyHDAC4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 451-650) antibodyHDAC4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (pSer632) antibodyHDAC4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 1049-1077), (C-Term) antibodyHDAC4 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB33667 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (pSer220), (pSer246), (pSer259) antibodyHDAC4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) antibodyHDAC4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each WB: 1 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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浓度
- 0.5 μg/μL
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缓冲液
- PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- 4 °C,-20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells." in: Journal of translational medicine, Vol. 14, pp. 7, (2016) (PubMed).
: "Tasquinimod Is an Allosteric Modulator of HDAC4 survival signaling within the compromised cancer microenvironment." in: Cancer research, Vol. 73, Issue 4, pp. 1386-99, (2013) (PubMed).
: "A limited group of class I histone deacetylases acts to repress human immunodeficiency virus type 1 expression." in: Journal of virology, Vol. 83, Issue 10, pp. 4749-56, (2009) (PubMed).
: "Histone deacetylase 7 silencing alters endothelial cell migration, a key step in angiogenesis." in: Circulation research, Vol. 101, Issue 12, pp. 1237-46, (2007) (PubMed).
: "Estrogen receptor alpha inhibits p53-mediated transcriptional repression: implications for the regulation of apoptosis." in: Cancer research, Vol. 67, Issue 16, pp. 7746-55, (2007) (PubMed).
: "Mechanisms of transcriptional repression of cell-cycle G2/M promoters by p63." in: Nucleic acids research, Vol. 34, Issue 3, pp. 928-38, (2006) (PubMed).
: "DNA damage promotes histone deacetylase 4 nuclear localization and repression of G2/M promoters, via p53 C-terminal lysines." in: The Journal of biological chemistry, Vol. 281, Issue 4, pp. 2347-57, (2006) (PubMed).
: "Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters." in: Molecular and cellular biology, Vol. 25, Issue 9, pp. 3737-51, (2005) (PubMed).
: "Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 30435-40, (2003) (PubMed).
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- HDAC4 (Histone Deacetylase 4 (HDAC4))
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别名
- HDAC4
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背景
- HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.
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分子量
- 140 kDa
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基因ID
- 9759
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NCBI登录号
- NP_006028
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process
抗原
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