Histone H2A, Idic Patch (AA 89-91) 抗体
There are 4+ publications for this product available. The 兔 多克隆 anti-Histone H2A, Idic Patch antibody is suitable to detect Histone H2A, Idic Patch in samples from 人. It has been validated for ChIP 和 WB.
(4 references)
产品编号 ABIN2668306
发货至:
中国
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Quick Overview for Histone H2A, Idic Patch (AA 89-91) 抗体 (ABIN2668306)
抗原
Histone H2A, Idic Patch
适用
人
宿主
兔
克隆类型
多克隆
应用范围
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
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抗原表位
- AA 89-91
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原理
- Histone H2A, acidic patch antibody (pAb)
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纯化方法
- Unpurified
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免疫原
- This Histone H2A, acidic patch antibody was raised against a peptide including amino acids 89-91 of histone H2A.
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应用备注
- ChIP: 10 µL per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Bookmarking promoters in mitotic chromatin: poly(ADP-ribose)polymerase-1 as an epigenetic mark." in: Nucleic acids research, Vol. 42, Issue 11, pp. 7028-38, (2014) (PubMed).
: "Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. ..." in: Nature communications, Vol. 5, pp. 4181, (2014) (PubMed).
: "A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex." in: Molecular and cellular biology, Vol. 33, Issue 8, pp. 1487-502, (2013) (PubMed).
: "Specific inhibition of NF-Y subunits triggers different cell proliferation defects." in: Nucleic acids research, Vol. 39, Issue 13, pp. 5356-68, (2011) (PubMed).
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- Histone H2A, Idic Patch
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背景
- Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.
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分子量
- 14 kDa
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基因ID
- 92815
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NCBI登录号
- NP_003508
抗原
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