DNMT3A 抗体
(3 references)Our Local Distributor
北京 101111
Quick Overview for DNMT3A 抗体 (ABIN2668262)
抗原
See all DNMT3A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- DNMT3A antibody (mAb) (Clone 64B1446)
-
纯化方法
- Protein G Chromatography
-
免疫原
- This DNMT3A antibody was raised against a full-length protein corresponding to mouse DNMT3A.
-
亚型
- IgG1 kappa
-
-
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody
DNMT3A 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB01852 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 500-700) antibodyDNMT3A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 26-100) antibodyDNMT3A 适用: 人, 小鼠, 大鼠 FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 46-180) antibodyDNMT3A 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 4G5F11 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) antibodyDNMT3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibodyDNMT3A 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 705-908), (C-Term) antibodyDNMT3A 适用: 人, 小鼠 WB, IHC, ICC, ChIP 宿主: 小鼠 Monoclonal 64B1446 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) antibodyDNMT3A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 31-61), (N-Term) antibodyDNMT3A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB15267 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 723-752), (C-Term) antibodyDNMT3A 适用: 人 WB 宿主: 兔 Polyclonal RB12504 unconjugated
-
-
应用备注
- Validated Applications: WB: 2 - 4 μg/mL dilution Individual optimization may be required. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. Published Applications: ChIP IHC See references for more information.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 μg/μL
-
缓冲液
- PBS containing 0.05 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
-
有效期
- 24 months
-
-
-
: "An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation." in: Nucleic acids research, Vol. 44, Issue 16, pp. 7605-17, (2016) (PubMed).
: "Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines." in: Biochemical pharmacology, Vol. 81, Issue 11, pp. 1329-42, (2011) (PubMed).
: "DNA methylation of colon mucosa in ulcerative colitis patients: correlation with inflammatory status." in: Inflammatory bowel diseases, Vol. 17, Issue 9, pp. 1955-65, (2011) (PubMed).
-
-
- DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))
-
别名
- DNMT3A
-
背景
- DNMT3A - DNA methyltransferase proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells.
-
分子量
- 116 kDa
-
基因ID
- 1788
-
NCBI登录号
- NP_001258682
抗原
-
