EIF2C3 抗体 (AA 1-175)
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Quick Overview for EIF2C3 抗体 (AA 1-175) (ABIN2668249)
抗原
See all EIF2C3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-175
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原理
- Ago3 antibody (mAb) (Clone 4B1-F6)
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纯化方法
- Protein G Chromatography
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免疫原
- This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.
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亚型
- IgG2a
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Ribozyme-enhanced single-stranded Ago2-processed interfering RNA triggers efficient gene silencing with fewer off-target effects." in: Nature communications, Vol. 6, pp. 8430, (2015) (PubMed).
: "Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 7964-9, (2008) (PubMed).
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- EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))
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别名
- Ago3
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背景
- Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.
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分子量
- 110 kDa
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基因ID
- 192669
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NCBI登录号
- NP_079128
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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