ISM1 抗体 (PE)
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北京 101111
Quick Overview for ISM1 抗体 (PE) (ABIN2662774)
抗原
See all ISM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
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亚型
- IgG2a kappa
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anti-Isthmin 1 (ISM1) antibody
ISM1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Isthmin 1 (ISM1) (AA 435-464) antibodyISM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB25991 unconjugated
anti-Isthmin 1 (ISM1) (AA 435-464), (C-Term) antibodyISM1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from prolonged exposure to light. Do not freeze.
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储存条件
- 4 °C
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储存方法
- The antibody solution should be stored undiluted between 2°C and 8°C.
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- ISM1 (Isthmin 1 (ISM1))
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别名
- Isthmin-1
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背景
- Isthmin is a secreted protein, and it was initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98 % identity between mouse and human, 87-88 % identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved with 99 % identity between mouse and human, 91 % identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in the presence of VEGF. Overexpression of isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk.
抗原
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