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TRA-1-60(R) 抗体 (Biotin)

This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, WB, IP, ICC 和 IF. Suitable for 人.
产品编号 ABIN2661341
发货至: 中国

Quick Overview for TRA-1-60(R) 抗体 (Biotin) (ABIN2661341)

抗原

TRA-1-60(R)

适用

宿主

  • 1
小鼠

克隆类型

  • 1
单克隆

标记

  • 1
Biotin

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

克隆位点

TRA-1-60-R
  • 纯化方法

    The solution is free of unconjugated biotin.

    亚型

    IgM kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.

    储存条件

    4 °C

    储存方法

    The antibody solution should be stored undiluted between 2°C and 8°C.
  • 抗原

    TRA-1-60(R)

    别名

    TRA-1-60-R

    背景

    TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.
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