TRA-1-60(R) 抗体 (Biotin)
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, WB, IP, ICC 和 IF. Suitable for 人.
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Quick Overview for TRA-1-60(R) 抗体 (Biotin) (ABIN2661341)
抗原
TRA-1-60(R)
适用
人
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
克隆位点
TRA-1-60-R
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纯化方法
- The solution is free of unconjugated biotin.
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亚型
- IgM kappa
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anti-TRA-1-60(R) antibody
适用: 人 WB 宿主: 小鼠 Monoclonal TRA-1-60(R) unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- The antibody solution should be stored undiluted between 2°C and 8°C.
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- TRA-1-60(R)
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别名
- TRA-1-60-R
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背景
- TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.
抗原
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