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FLI1 抗体

The 兔 多克隆 anti-FLI1 antibody (ABIN265422) specifically detects FLI1 in WB 和 IHC (p). The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN265422
发货至: 中国
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Quick Overview for FLI1 抗体 (ABIN265422)

抗原

See all FLI1 抗体
FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

适用

  • 116
  • 48
  • 22
  • 9
  • 6
  • 6
  • 6
  • 6
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
人, 小鼠

宿主

  • 81
  • 35
  • 2

克隆类型

  • 66
  • 52
多克隆

标记

  • 78
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This FLI1 antibody is un-conjugated

应用范围

  • 92
  • 48
  • 33
  • 29
  • 19
  • 12
  • 10
  • 8
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of FLI1 protein. (region surrounding Arg319)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    纯度

    > 95 % pure by SDS-PAGE
  • 应用备注

    ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

    别名

    FLI1

    背景

    Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.Synonyms: ERGB transcription factor, Fli-1 proto-oncogene, Friend leukemia integration 1 transcription factor, vascular tumor marker

    分子量

    approx. 50 kDa

    基因ID

    2313

    NCBI登录号

    NP_002008

    UniProt

    Q01543
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