E2F4/E2F5 (E2F4/5) 抗体
The 兔 多克隆 anti-E2F4/E2F5 antibody (ABIN265389) specifically detects E2F4/E2F5 in WB, IHC (p) 和 EIA.
The antibody is reactive with 人, 小鼠 和 大鼠 samples.
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产品编号 ABIN265389
发货至:
中国
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中国
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北京 101111
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Quick Overview for E2F4/E2F5 (E2F4/5) 抗体 (ABIN265389)
抗原
See all E2F4/E2F5 (E2F4/5) 抗体
E2F4/E2F5 (E2F4/5)
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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特异性
- This antibody detects endogenous levels of E2F4 / E2F5 protein (region surrounding Leu50/83).
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-E2F4/E2F5 (E2F4/5) (Internal Region) antibody
E2F4/5 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- E2F4/E2F5 (E2F4/5)
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别名
- E2F4/E2F5
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背景
- The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an Synonyms: E2F-4, E2F-5, Transcription Factor E2F4, Transcription Factor E2F5
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分子量
- approx. 44 kDa
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途径
- Mitotic G1-G1/S Phases
抗原
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