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DP1 抗体

This 兔 多克隆 antibody specifically detects DP1 in WB, IF, IHC (p) 和 EIA. It exhibits reactivity toward 人.
产品编号 ABIN265387
发货至: 中国
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Quick Overview for DP1 抗体 (ABIN265387)

抗原

See all DP1 (TFDP1) 抗体
DP1 (TFDP1) (Transcription Factor Dp-1 (TFDP1))

适用

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宿主

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克隆类型

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多克隆

标记

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This DP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody detects endogenous levels of DP-1/TFDP1 protein (region surrounding Val393).

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

    纯度

    > 95 % pure by SDS-PAGE
  • 应用备注

    ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    DP1 (TFDP1) (Transcription Factor Dp-1 (TFDP1))

    别名

    TFDP1

    背景

    The human retinoblastoma gene product appears to play an important role in the negative egulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 forms heterodimers with a second protein, designated DP-1, forming an Synonyms: DP1, DRTF1, DRTF1-polypeptide 1, E2F dimerization partner 1, Transcription factor Dp-1

    分子量

    approx. 55 kDa

    基因ID

    7027

    NCBI登录号

    NP_009042

    UniProt

    Q14186

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases
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