Actin 抗体 (pan)
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北京 101111
Quick Overview for Actin 抗体 (pan) (ABIN265298)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pan
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特异性
- This antibody detects endogenous levels of pan Actin (region surrounding Glu361).
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen
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anti-Actin antibody
适用: 植物 WB 宿主: 小鼠 Monoclonal Q30 unconjugated
anti-Actin (AA 165-260) antibody适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 犬, Cow, Pig, 豚鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 300-380) antibody适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 小鸡 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Actin antibody适用: Arabidopsis, Oryza sativa, Nicotiana tabacum, Barley WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 367-377) antibody适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 21-70) antibody适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 189-218) antibody适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Actin
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背景
- All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
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分子量
- approx. 45.0 kDa
抗原
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