Western Blot: detects recombinant Rit protein expressed in HEK-293 cells, Shows minimalcross-reactivity with Rin protein and other Ras proteins. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
Phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
NS8 antibody, RIBB antibody, RIT antibody, ROC1 antibody, RIT1 antibody, rit1 antibody, MGC79097 antibody, RGD1559874 antibody, si:ch211-93a2.3 antibody, Rit antibody, Ras like without CAAX 1 antibody, Ras-like without CAAX 1 L homeolog antibody, Ras-like without CAAX 1 antibody, RIT1 antibody, rit1.L antibody, Rit1 antibody, rit1 antibody
背景
Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.Synonyms: RIBB, RIT, ROC1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1