Farnesyl 抗体
IF, EIA
宿主: 兔
Polyclonal
unconjugated
1 image
-
- 抗原
- Farnesyl
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
- 应用范围
- Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 纯化方法
- Purified
- 免疫原
- Antibody developed using Farnesyl cysteine conjugated to KLH.
- 亚型
- IgG
-
anti-Farnesyl antibody
IF, ELISA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Western Blot: Detects farnesyl motif derived from isoprenylated C-A-A-X sequence. Alsocross-reacts with geranylgeranyl motif. Detects KLH as well. Not suitable for Western blotdue to hydrophobicity of isoprenylated motif.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline with 0.08 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
-
- 抗原
- Farnesyl
- 物质类
- Chemical
- 背景
- Protein isoprenylation is a post-translational modification that affects about 0.5?% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A 1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.
-
You are here:
