ACP1 抗体
Quick Overview for ACP1 抗体 (ABIN264957)
抗原
See all ACP1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts to LMW Protein Tyrosine Phosphatase.
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纯化方法
- Purified
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免疫原
- The human protein, HCPTPA was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
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亚型
- IgG
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anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibody
ACP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A3 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 15-144) antibodyACP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 33-61), (N-Term) antibodyACP1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB23949 unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 1-158) antibodyACP1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) antibodyACP1 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IP, IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 21-70) antibodyACP1 适用: 人, Cow, 兔, 马, 猴, 小鸡, Drosophila melanogaster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (AA 33-61) antibodyACP1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Acid Phosphatase 1, Soluble (ACP1) (Middle Region) antibodyACP1 适用: 人, 大鼠, 小鼠, Cow, Pig, 山羊, 豚鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot. Immunoprecipitation: 1 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS, 0.08 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
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- ACP1 (Acid Phosphatase 1, Soluble (ACP1))
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别名
- ACP1 / LMW-PTPase
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背景
- Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinylphosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1) 2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.Synonyms: Adipocyte acid phosphatase, HAAP, Low molecular weight cytosolic acid phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Red cell acid phosphatase 1
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基因ID
- 52
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NCBI登录号
- NP_004291
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UniProt
- P24666
抗原
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