Uromodulin 抗体
Quick Overview for Uromodulin 抗体 (ABIN264838)
抗原
See all Uromodulin (UMOD) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody gives a single arc when tested by IEP against fresh urine. No arcs are visible when tested by IEP against serum. Identity has been confirmed by double diffusion (Ouchterlony) against uromucoid and a known anti-uromucoid.
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纯化方法
- Ion exchange chromatography
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免疫原
- Human Uromucoid, purified from human urine
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应用备注
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The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. RID: 10l antiserum/cm2 in gel vs 5l uromucoid - 50 mg/L Neat - 1: 5. Double Diffusion: 10l antiserum vs 3l uromucoid - 50 mg/LIEP: 100l antiserum vs 5 μL uromucoid - 50 mg/L
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 14.8mg/mL (OD280nm E1% = 14.5)
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缓冲液
- Glycine buffered saline, pH 7.4 containing 0.09 % Sodium azide, 0.1 % E-Amino-n-Caproic acid, 0.01 % Benzamidine, 1 mM EDTA
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储存液
- Sodium azide, Benzamidine, EACA
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注意事项
- This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- Uromodulin (UMOD)
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背景
- Synonyms: THP, Tamm-Horsfall urinary glycoprotein
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基因ID
- 7369
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NCBI登录号
- NP_001008390
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UniProt
- P07911
抗原
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