Malondialdehyde 抗体 (HRP)
Quick Overview for Malondialdehyde 抗体 (HRP) (ABIN264632)
抗原
See all Malondialdehyde (MDA) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- MDA modified protein-Sepharose affinity column and conjugated with HRP
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应用备注
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ELISA. Western Blot: 1: 1,000 - 1: 4,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 0.5 mL distilled water. Centrifuge if not completely clear after standing for 1-2 hours at room temperature.
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浓度
- 1.2mg/mL (prior to lyophilization)
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缓冲液
- Lyophilized from 50 mM PBS, 0.1 M Sodium Chloride, pH 7.4 containing 10 mg/mL BSA containing 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- Malondialdehyde (MDA)
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别名
- Malondialdehyde / MDA
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物质类
- Chemical
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背景
- Malondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.Synonyms: 1, 3-Propanedial, 3-Propanedione, Malondialdehyde Malonyldialdehyde, Malonic aldehyde, Malonic dialdehyde, Malonodialdehyde, Propanedial
抗原
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