Carboxy Methyl Lysine 抗体 (methylated)
This anti-Carboxy Methyl Lysine antibody is a 山羊 多克隆 antibody detecting Carboxy Methyl Lysine in EIA 和 WB. Suitable for .
Quick Overview for Carboxy Methyl Lysine 抗体 (methylated) (ABIN264528)
抗原
See all Carboxy Methyl Lysine (CML) 抗体
Carboxy Methyl Lysine (CML)
宿主
山羊
克隆类型
多克隆
标记
This Carboxy Methyl Lysine antibody is un-conjugated
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
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抗原表位
- methylated
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纯化方法
- CML-protein Sepharose affinity chromatography
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免疫原
- Carboxymethylated Keyhole Limpet Hemocyanin (CML-KLH). Prepared by the reaction of the protein with glyoxylic acid.
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应用备注
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Western Blot. ELISA: 1: 4,000 - 1: 20,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL (OD280nm, E0.1% = 1.35)
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缓冲液
- 75 mM PBS, pH 7.2 containing 75 mM Sodium chloride and 0.5 mM EDTA and 0.02 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Carboxy Methyl Lysine (CML)
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别名
- Carboxy Methyl Lysine
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物质类
- Amino Acid
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背景
- Carboxymethyl Lysine is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.Synonyms: CML, Carboxymethyl-lysine
抗原
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