Myogenin 抗体 (AA 30-224)
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Quick Overview for Myogenin 抗体 (AA 30-224) (ABIN264431)
抗原
See all Myogenin (MYOG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 30-224
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特异性
- Recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
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产品特性
- Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3, Class C basic helix-loop-helix protein 3
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纯化方法
- Purified
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免疫原
- Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein containing rat myogenin amino acids 30-224 and mouse myelomaNS1 cells. Genename: MYOG
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亚型
- IgG1
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应用备注
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ELISA. Gel Supershift. Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 μg/mL). Immunofluorescence. Immunoprecipitation (2 μg/mL). Western Blot (1 μg/mL). Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.08 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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别名
- Myogenin
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背景
- Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4
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基因ID
- 29148
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UniProt
- P20428
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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