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Myogenin 抗体 (AA 30-224)

This 小鼠 单克隆 anti-Myogenin antibody (Clone F12B) specifically detects Myogenin in WB, IF, IP, IHC (p), EIA 和 EMSA. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN264431
发货至: 中国
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Quick Overview for Myogenin 抗体 (AA 30-224) (ABIN264431)

抗原

See all Myogenin (MYOG) 抗体
Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

适用

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  • 1
人, 小鼠, 大鼠

宿主

  • 72
  • 63
  • 1
小鼠

克隆类型

  • 75
  • 61
单克隆

标记

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This Myogenin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

克隆位点

F12B
  • 抗原表位

    • 30
    • 16
    • 13
    • 13
    • 10
    • 8
    • 8
    • 5
    • 3
    • 3
    • 2
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    AA 30-224

    特异性

    Recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.

    产品特性

    Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3, Class C basic helix-loop-helix protein 3

    纯化方法

    Purified

    免疫原

    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein containing rat myogenin amino acids 30-224 and mouse myelomaNS1 cells. Genename: MYOG

    亚型

    IgG1
  • 应用备注

    ELISA. Gel Supershift. Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 μg/mL). Immunofluorescence. Immunoprecipitation (2 μg/mL). Western Blot (1 μg/mL). Positive Control: Rhabdomyosarcoma and Wlim's tumors.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 缓冲液

    PBS containing 0.08 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    有效期

    12 months
  • 抗原

    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

    别名

    Myogenin

    背景

    Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

    基因ID

    29148

    UniProt

    P20428

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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