MITF 抗体
(4 references)Quick Overview for MITF 抗体 (ABIN264430)
抗原
See all MITF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes in Western blotting a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor. This antibody does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.does not
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产品特性
- Synonyms: Microphthalmia-associated transcription factor, Mi-protein
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纯化方法
- Purified
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免疫原
- Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with anN-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. Genename: MITF
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亚型
- IgG1
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anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 351-450) antibody
MITF 适用: 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer180), (pSer73) antibodyMITF 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibodyMITF 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 8F1G5 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-28), (N-Term) antibodyMITF 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18810 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人, 犬 IHC, IF, FACS, StM 宿主: 兔 Monoclonal MITF-2987R unconjugated Recombinant Antibody
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibodyMITF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D5 unconjugated
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应用备注
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Gel Supershift. Western Blot (1 μg/mL). Immunoprecipitation (2 μg/mg of protein lysate). Immunohistochemistry (Frozen and Paraffin Sections). Positive Control: 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclastcells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.08 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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: "Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells." in: Journal of biophotonics, Vol. 8, Issue 6, pp. 466-79, (2016) (PubMed).
: "Canonical Wnt/?-catenin signalling is essential for optic cup formation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81158, (2013) (PubMed).
: "EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 600-10, (2013) (PubMed).
: "Self-formation of optic cups and storable stratified neural retina from human ESCs." in: Cell stem cell, Vol. 10, Issue 6, pp. 771-85, (2012) (PubMed).
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: "Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells." in: Journal of biophotonics, Vol. 8, Issue 6, pp. 466-79, (2016) (PubMed).
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- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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别名
- MITF
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背景
- There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3Synonyms: Mi-protein, Microphthalmia-associated transcription factor
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分子量
- 52-56 kDa
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基因ID
- 4286
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UniProt
- O75030
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途径
- Chromatin Binding
抗原
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