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CAPN1 抗体

This 小鼠 单克隆 antibody specifically detects CAPN1 in WB, IHC (p), IP 和 EIA. It exhibits reactivity toward 人, 小鼠, 大鼠 和 Cow.
产品编号 ABIN264388
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CAPN1 抗体 (ABIN264388)

抗原

See all CAPN1 抗体
CAPN1 (Calpain 1 (CAPN1))

适用

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人, 小鼠, 大鼠, Cow

宿主

  • 69
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小鼠

克隆类型

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单克隆

标记

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This CAPN1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

克隆位点

15C10
  • 特异性

    This antibody (large subunit, p80) recognizes the 80 kDa subunit of μ-Calpain as well as two smaller proteins that are presumed to be autolysis products.

    产品特性

    Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit,Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cellproliferation-inducing gene 30 protein

    纯化方法

    Standard chromatographic techniques.

    免疫原

    Calpain purified from human placenta. Genename: CAPN1

    亚型

    IgG1
  • 应用备注

    ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 缓冲液

    20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    有效期

    12 months
  • 抗原

    CAPN1 (Calpain 1 (CAPN1))

    别名

    Calpain-1

    背景

    The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, PIG30, muCANP

    基因ID

    823

    UniProt

    P07384
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