CAPN1 抗体
Quick Overview for CAPN1 抗体 (ABIN264388)
抗原
See all CAPN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody (large subunit, p80) recognizes the 80 kDa subunit of μ-Calpain as well as two smaller proteins that are presumed to be autolysis products.
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产品特性
- Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit,Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cellproliferation-inducing gene 30 protein
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纯化方法
- Standard chromatographic techniques.
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免疫原
- Calpain purified from human placenta. Genename: CAPN1
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亚型
- IgG1
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anti-Calpain 1 (CAPN1) (AA 625-713) antibody
CAPN1 适用: 人, 小鼠, 大鼠 WB, FACS, IP, IHC (p), IF (cc), IF (p), ELISA, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) antibodyCAPN1 适用: 人 WB, ICC, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) antibodyCAPN1 适用: 人, 小鼠, 大鼠, Cow WB, IHC, IP, IHC (p), ELISA 宿主: 小鼠 Monoclonal 15C10 unconjugated
anti-Calpain 1 (CAPN1) (AA 515-526) antibodyCAPN1 适用: 人 WB, IHC, IHC (p), ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) (AA 185-234) antibodyCAPN1 适用: 人, Cow, Pig, 山羊, 兔, 绵羊, Bat, 小鸡, 犬, 豚鼠, 马, 猴, 非洲爪蟾, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) (AA 300-349) antibodyCAPN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 山羊, 兔, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- CAPN1 (Calpain 1 (CAPN1))
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别名
- Calpain-1
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背景
- The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, PIG30, muCANP
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基因ID
- 823
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UniProt
- P07384
抗原
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