Calpain 抗体
This anti-Calpain antibody is a 小鼠 单克隆 antibody detecting Calpain in WB, IP 和 EIA. Suitable for 人 和 Cow.
Quick Overview for Calpain 抗体 (ABIN264387)
抗原
Calpain
适用
人, Cow
宿主
小鼠
克隆类型
单克隆
标记
This Calpain antibody is un-conjugated
应用范围
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
克隆位点
28F3
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产品特性
- Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1
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纯化方法
- Standard chromatographic techniques.
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免疫原
- Calpain purified from human placenta.
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亚型
- IgG1
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anti-Calpain (AA 396-555), (Catalytic Subunit) antibody
适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calpain (AA 1-19) antibody适用: 人 WB 宿主: 小鼠 Monoclonal Calp3d-2C4 unconjugated
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应用备注
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ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- 0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as presevtative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- Calpain
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背景
- The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2
抗原
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