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Calpain 抗体 (Agarose Beads)

The 小鼠 单克隆 anti-Calpain antibody is suitable to detect Calpain in samples from 人. It has been validated for IP.
产品编号 ABIN264386
发货至: 中国
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Quick Overview for Calpain 抗体 (Agarose Beads) (ABIN264386)

抗原

Calpain

适用

  • 4
  • 3
  • 1
  • 1
  • 1

宿主

  • 4
  • 3
小鼠

克隆类型

  • 4
  • 3
单克隆

标记

  • 5
  • 1
  • 1
This Calpain antibody is conjugated to Agarose Beads

应用范围

  • 7
  • 4
  • 3
  • 1
  • 1
Immunoprecipitation (IP)

克隆位点

28F3
  • 产品特性

    Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1

    纯化方法

    Standard chromatographic techniques.

    免疫原

    Calpain purified from human placenta.

    亚型

    IgG1
  • 应用备注

    ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    有效期

    12 months
  • 抗原

    Calpain

    背景

    The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2
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