ITGAL 抗体 (Biotin)
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- 抗原 See all ITGAL 抗体
- ITGAL (Integrin alpha-L (ITGAL))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGAL antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with LFA-1 alpha. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
- 产品特性
- Synonyms: Integrin alpha-L, LFA1, LFA-1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocytefunction-associated molecule 1 alpha chain, CD11 antigen-like family member A
- 克隆位点
- OFYS
- 亚型
- IgG2a
- Top Product
- Discover our top product ITGAL Primary Antibody
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anti-Integrin alpha-L (ITGAL) (AA 26-306) antibody
ITGAL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) (AA 26-194) antibodyITGAL 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5G7C5 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, IHC 宿主: 小鼠 Monoclonal 38 unconjugated
anti-Integrin alpha-L (ITGAL) (AA 201-500) antibodyITGAL 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal M17-4 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibody (Biotin)ITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 Biotin
anti-Integrin alpha-L (ITGAL) antibody (PE)ITGAL 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-25 PE
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- 应用备注
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Flow Cytometry. Characterization of LFA-1 immunodeficiency. Monitoring of subsets in peripheral blood. Graft-versus-host disease. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
- 限制
- 仅限研究用
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- 缓冲液
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. - 有效期
- 12 months
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- 抗原
- ITGAL (Integrin alpha-L (ITGAL))
- 别名
- CD11a / ITGAL (ITGAL 产品)
- 背景
- CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain
- 基因ID
- 3683
- UniProt
- P20701
- 途径
- Activated T Cell Proliferation, Integrin Complex
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