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CD28 抗体 (APC)

CD28 适用: 人 FACS 宿主: 小鼠 Monoclonal B-23 APC
产品编号 ABIN264248
发货至: 中国
  • 抗原 See all CD28 抗体
    CD28
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    小鼠
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    This CD28 antibody is conjugated to APC
    应用范围
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    Flow Cytometry (FACS)
    特异性
    This antibody recats with CD28 receptor.
    产品特性
    Synonyms: TP44, T-cell-specific surface glycoprotein CD28
    克隆位点
    B-23
    亚型
    IgG1
    Top Product
    Discover our top product CD28 Primary Antibody
  • 应用备注
    Flow Cytometry. Monitoring of activated T cells in peripheral blood. Study of T lymphocyte cytokine function. Study of B cell activation. Study of Cell-adhesion molecules relating T and B lymphocytes.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.
    限制
    仅限研究用
  • 缓冲液
    PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store the antibody undiluted at 2-8 °C. Do Not Freeze!
    Shelf life: One year from despatch.
    有效期
    12 months
  • 抗原
    CD28
    别名
    CD28 (CD28 产品)
    别名
    Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
    背景
    Anti-human CD28 binds the 44 kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
    基因ID
    940
    UniProt
    P10747
    途径
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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