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HSP70 抗体

Name: HSP70 抗体
SKU: ABIN263937
Availability: OutOfStock

HSP70 适用: 人, 小鼠, 大鼠, 小鸡, Drosophila melanogaster, Cow, Pig, 猴, 犬, Hamster, 绵羊, 豚鼠, 兔, C. elegans, Carp WB, IP, IF, FACS, IEM, IHC (fro), EIA 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated

Western Blotting (WB) image for anti-Heat Shock Protein 70 (HSP70) antibody (ABIN263937) (HSP70 抗体)

Immunohistochemistry (IHC) image for anti-Heat Shock Protein 70 (HSP70) antibody (ABIN263937) (HSP70 抗体)

IHC

3 images

(31 references)

产品编号 ABIN263937

发货至: 中国

Datasheet as PDF

抗原 See all HSP70 抗体

HSP70 (Heat Shock Protein 70 (HSP70))
适用
306

188

154

64

60

53

52

52

36

31

29

29

28

23

20

18

15

13

12

10

10

9

9

9

9

7

6

5

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 小鸡, Drosophila melanogaster, Cow, Pig, 猴, 犬, Hamster, 绵羊, 豚鼠, 兔, C. elegans, Carp
宿主
198

135

13

11

3
小鼠
Compatible Secondaries
克隆类型
212

147
单克隆
标记
105

30

26

24

21

19

18

15

14

14

10

5

5

5

5

5

5

5

5

4

4

4

3

3

2

1

1

1

1
This HSP70 antibody is un-conjugated
应用范围
344

143

135

110

109

108

94

66

62

61

41

29

22

21

21

6

4

2

2

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoelectron Microscopy (IEM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

特异性

This antibody Detects a ~70 kDa protein corresponding to the Molecular Mass of inducible HSP70 on SDS PAGE Immunoblots. The mapped epitope is in the region of amino acid residues 436-503. Does not cross-react with HSC70 (HSP73).

产品特性

Synonyms: HSP70.1, HSP70-1/HSP70-2, HSPA1A, HSPA1B, HSPA1, Heat shock 70 kDa protein 1A/1B

纯化方法

Affinity Chromatography on Protein G

免疫原

Human HSP70 (1)

克隆位点

C92F3A-5

亚型

IgG1

Top Product

Discover our top product HSP70 Primary Antibody
anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, FACS, ICC, IEM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody (HRP)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Heat Shock Protein 70 (HSP70) antibody (FITC)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal FITC

anti-Heat Shock Protein 70 (HSP70) antibody (Alkaline Phosphatase (AP))
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal Biotin

anti-Heat Shock Protein 70 (HSP70) antibody (PE)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal PE

anti-Heat Shock Protein 70 (HSP70) antibody (APC)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal APC

anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal PerCP

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)
HSP70 适用: 人 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal Atto 488

应用备注

ELISA (10). Western blot (7,8,14)1 μg/mL was sufficient for detection of HSP70 in 20 μg of HeLa lysate by colorimetric

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Shelf life: one year from despatch.

有效期

12 months
Ishikawa, Sakurai: "Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells." in: The FEBS journal, Vol. 282, Issue 2, pp. 332-40, (2015) (PubMed).

Katoh, Kubota, Kita, Nakatsu, Aoki, Mori, Maenaka, Takeda, Kidokoro: "Heat shock protein 70 regulates degradation of the mumps virus phosphoprotein via the ubiquitin-proteasome pathway." in: Journal of virology, Vol. 89, Issue 6, pp. 3188-99, (2015) (PubMed).

Sheppard, Sun, Khammash, Giffard: "Overexpression of heat shock protein 72 attenuates NF-?B activation using a combination of regulatory mechanisms in microglia." in: PLoS computational biology, Vol. 10, Issue 2, pp. e1003471, (2014) (PubMed).

Akkad, Corpeno, Larsson: "Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model." in: PLoS ONE, Vol. 9, Issue 4, pp. e92622, (2014) (PubMed).

Richter, Viergutz, Schwerin, Weitzel: "Prostaglandin E synthase interacts with inducible heat shock protein 70 after heat stress in bovine primary dermal fibroblast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 87, Issue 1, pp. 61-7, (2014) (PubMed).

Dang, Tanabe, Tanaka, Tokumoto, Misumi, Saeki, Fujikuni, Ohdan: "Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation." in: PLoS ONE, Vol. 9, Issue 10, pp. e110748, (2014) (PubMed).

Wijeratne, Xu, Scherz-Shouval, Marron, Rocha, Liu, Costa-Lotufo, Santagata, Lindquist, Whitesell, Gunatilaka: "Structure-activity relationships for withanolides as inducers of the cellular heat-shock response." in: Journal of medicinal chemistry, Vol. 57, Issue 7, pp. 2851-63, (2014) (PubMed).

Eroglu, Kimbler, Pang, Choi, Moskophidis, Yanasak, Dhandapani, Mivechi: "Therapeutic inducers of the HSP70/HSP110 protect mice against traumatic brain injury." in: Journal of neurochemistry, Vol. 130, Issue 5, pp. 626-41, (2014) (PubMed).

Seguin, Morelli, Vinet, Amore, De Biasi, Poletti, Rubinsztein, Carra: "Inhibition of autophagy, lysosome and VCP function impairs stress granule assembly." in: Cell death and differentiation, Vol. 21, Issue 12, pp. 1838-51, (2014) (PubMed).

Aare, Radell, Eriksson, Akkad, Chen, Hoffman, Larsson: "Effects of corticosteroids in the development of limb muscle weakness in a porcine intensive care unit model." in: Physiological genomics, Vol. 45, Issue 8, pp. 312-20, (2013) (PubMed).

Bauckman, Haller, Flores, Nanjundan: "Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells." in: Cell death & disease, Vol. 4, pp. e592, (2013) (PubMed).

Morshed, Ma, Latif, Davies: "How one TSH receptor antibody induces thyrocyte proliferation while another induces apoptosis." in: Journal of autoimmunity, Vol. 47, pp. 17-24, (2013) (PubMed).

Larkins, Murphy, Lamb: "Influences of temperature, oxidative stress, and phosphorylation on binding of heat shock proteins in skeletal muscle fibers." in: American journal of physiology. Cell physiology, Vol. 303, Issue 6, pp. C654-65, (2012) (PubMed).

Abisambra, Jinwal, Suntharalingam, Arulselvam, Brady, Cockman, Jin, Zhang, Dickey: "DnaJA1 antagonizes constitutive Hsp70-mediated stabilization of tau." in: Journal of molecular biology, Vol. 421, Issue 4-5, pp. 653-61, (2012) (PubMed).

Santagata, Xu, Wijeratne, Kontnik, Rooney, Perley, Kwon, Clardy, Kesari, Whitesell, Lindquist, Gunatilaka: "Using the heat-shock response to discover anticancer compounds that target protein homeostasis." in: ACS chemical biology, Vol. 7, Issue 2, pp. 340-9, (2012) (PubMed).

Chanoux, Robay, Shubin, Kebler, Suaud, Rubenstein: "Hsp70 promotes epithelial sodium channel functional expression by increasing its association with coat complex II and its exit from endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 287, Issue 23, pp. 19255-65, (2012) (PubMed).

Zhang, Ahn, Benjamin, Honda, Hicks, Calabrese, Cole, Dinkova-Kostova: "HSF1-dependent upregulation of Hsp70 by sulfhydryl-reactive inducers of the KEAP1/NRF2/ARE pathway." in: Chemistry & biology, Vol. 18, Issue 11, pp. 1355-61, (2012) (PubMed).

Mitsuhashi, Yamaguchi, Kojima, Nakajima, Kasai: "Effects of HSP70 on the compression force-induced TNF-? and RANKL expression in human periodontal ligament cells." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 60, Issue 2, pp. 187-94, (2011) (PubMed).

Batista-Nascimento, Neef, Liu, Rodrigues-Pousada, Thiele: "Deciphering human heat shock transcription factor 1 regulation via post-translational modification in yeast." in: PLoS ONE, Vol. 6, Issue 1, pp. e15976, (2011) (PubMed).

Shipp, Watson, Jones: "Associations of HSP90 client proteins in human breast cancer." in: Anticancer research, Vol. 31, Issue 6, pp. 2095-101, (2011) (PubMed).

show more references
抗原

HSP70 (Heat Shock Protein 70 (HSP70))

别名

Heat Shock Protein 70 / HSP70 (HSP70 产品)

别名

APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody

背景

Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.Synonyms: HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B

基因ID

3303

UniProt

P08107