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CD69 抗体

Name: CD69 抗体
SKU: ABIN263925
Availability: OutOfStock

Cited in 6+ publications. This anti-CD69 antibody is a 小鼠单克隆 antibody detecting CD69 in FACS, WB, IHC (fro) 和 Func. Suitable for 人.

FACS

1 image

(6 references)

产品编号 ABIN263925

发货至: 中国

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Quick Overview for CD69 抗体 (ABIN263925)

抗原

See all CD69 抗体

CD69

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This CD69 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

克隆位点

FN50

Discover our top product CD69 Primary Antibody

特异性

The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions.

产品特性

Synonyms: GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM, Early T-cell activation antigen p60, Earlyactivation antigen CD69, Activation inducer molecule, C-type lectin domain family 2member C

纯化方法

Affinity Chromatography on Protein A

免疫原

Anti-µ-stimulated human B lymphocytes

亚型

IgG1
anti-CD69 (CD69) (AA 101-199) antibody
CD69 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD69 (CD69) antibody
CD69 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8B6 unconjugated

anti-CD69 (CD69) (AA 1-199) antibody
CD69 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated

anti-CD69 (CD69) antibody
CD69 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal FN50 unconjugated

anti-CD69 (CD69) (AA 62-199) antibody
CD69 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD69 (CD69) (Internal Region) antibody
CD69 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD69 (CD69) antibody (PE)
CD69 适用: 人, Baboon, Cynomolgus, Macaque, 猕猴, Chimpanzee FACS 宿主: 小鼠 Monoclonal FN50 PE

anti-CD69 (CD69) (AA 90-199) antibody
CD69 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 4H3 unconjugated

anti-CD69 (CD69) antibody (PerCP)
CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 PerCP

anti-CD69 (CD69) (AA 119-148) antibody
CD69 适用: 人 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Flow Cytometry: 5 μg/mL. Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa. Immunohistochemistry on Frozen Sections. Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together withTPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 tostimulated peripheral blood B cells has similar, but weaker effect.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS with 15 mM Sodium Azide, pH ~7.4

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

有效期

12 months
Zawada, Mrak, Biedermann, Palmer, Gentleman, Aboud, Griffin et al.: "Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related ..." in: Acta neuropathologica communications, Vol. 3, pp. 9, (2015) (PubMed).

Johnson, Zhang, Adams, Phillips, Freitas, Froehner, Green-Church, Montanaro: "Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins." in: PLoS ONE, Vol. 7, Issue 8, pp. e43515, (2012) (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Krämer, Kess, Kremmer, Schulte-Hostede: "Multi-parameter determination of TNF?, PCT and CRP for point-of-care testing." in: The Analyst, Vol. 136, Issue 4, pp. 692-5, (2011) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, (2007) (PubMed).
抗原

CD69

别名

CD69

背景

CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.Synonyms: AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leu-23, MLR-3

基因ID

969

UniProt

Q07108