Bovine Coronavirus 抗体
This anti-Bovine Coronavirus antibody is a 小鼠 单克隆 antibody detecting Bovine Coronavirus in EIA. Suitable for Bovine Coronavirus (BCoV).
Quick Overview for Bovine Coronavirus 抗体 (ABIN263581)
抗原
Bovine Coronavirus (BCoV)
适用
Bovine Coronavirus (BCoV)
宿主
小鼠
克隆类型
单克隆
标记
This Bovine Coronavirus antibody is un-conjugated
应用范围
Enzyme Immunoassay (EIA)
克隆位点
5A4
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产品特性
- Synonyms: Spike glycoprotein, S glycoprotein
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纯化方法
- Protein G chromatography
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纯度
- > 95 % pure (SDS-PAGE)
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免疫原
- Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/cmice.
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亚型
- IgG1
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应用备注
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ELISA. HIT (hemagglutination inhibition titer).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.8mg/mL (OD280nm E0.1%=1.4)
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch. -
有效期
- 12 months
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- Bovine Coronavirus (BCoV)
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物质类
- Virus
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背景
- Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Synonyms: E2 protein, Peplomer protein, S glycoprotein, Spike glycoprotein
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UniProt
- P25193
抗原
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