Nucleostemin 抗体 (AA 115-126)
Quick Overview for Nucleostemin 抗体 (AA 115-126) (ABIN2613434)
抗原
See all Nucleostemin (GNL3) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 115-126
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原理
- GNL3 (aa115-126)
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序列
- TENKAKSGKQ NS
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特异性
- This antibody is expected to recognize both reported isoforms (NP_055181.3, NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5 μg/mL.
Western Blot: Approx 65 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 62.0 kDa according to NP_055181.3). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))
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别名
- GNL3
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背景
- GNL3, guanine nucleotide binding protein-like 3 (nucleolar), C77032, E2IG3, NNP47, NS, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide-binding protein-like 3, novel nucleolar protein 47, nucleolar GTP-binding pr
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基因ID
- 26354
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NCBI登录号
- NP_055181, NP_996561
抗原
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