The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
免疫原
PLD1 (phospho Thr147) antibody was raised against a peptide sequence around phosphorylation site of threonine 147 (R-H-T (p)-F-R) derived from Human PLD1.
Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.