(1 reference)-
- 抗原
- Fucose
- 适用
- Higher Plants
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunolocalization (IL), Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- 预测反应
- higher plants
- 纯化方法
- serum
- 免疫原
- core fucose residues bound to the N-glycan in alpha 1,3
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- 应用备注
- 1 μg/10 mL of incubation buffer, with standard ECL (WB), 1: 40 (IL)
- 说明
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controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 μL of sterile water.
- 缓冲液
- PBS pH 7.4
- 注意事项
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).
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: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).
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- 抗原
- Fucose
- 背景
- This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
- 分子量
- 10 - 100 for various glycoproteins
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