Fucose 抗体
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北京 101111
Quick Overview for Fucose 抗体 (ABIN249420)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-Fucose
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交叉反应 （详细）
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Higher plants, Pheodactylum tricornutum (diatom)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Higher plants,Phaeodactylum tricornutum
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纯化方法
- Immunogen affinity purified serum
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免疫原
- Core fucose residues bound to the N-glycan in alpha 1
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应用备注
- 0.5 μg/mL (ELISA), 1 : 40 (IL), 1 μg/10 mL (WB)
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说明
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controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." in: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).
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- Fucose
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背景
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Background: This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Additional information: Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc,) , However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants, This sugar residue does not exist in mammals, in their endogenous glycoproteins
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分子量
- 10 - 100 for various glycoproteins
抗原
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